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Eukaryotic Cell, December 2002, p. 1021-1031, Vol. 1, No. 6
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.6.1021-1031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rvs161p and Sphingolipids Are Required for Actin Repolarization following Salt Stress

Axelle Balguerie,1 Michel Bagnat,2 Marc Bonneu,1 Michel Aigle,1 and Annick M. Breton1*

Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, F-33077 Bordeaux Cedex, France,1 Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany2

Received 15 July 2002/ Accepted 29 September 2002

In Saccharomyces cerevisiae, the actin cytoskeleton is depolarized by NaCl stress. In this study, the response was maximal after 30 min, and then actin patches repolarized. Rvs161p was required for actin repolarization because the rvs161{Delta} mutant did not repolarize actin patches after growth in a salt medium. Mutations suppressing the rvs161{Delta}-related salt sensitivity all occurred in genes required for sphingolipid biosynthesis: FEN1, SUR4, SUR2, SUR1, and IPT1. These suppressors also suppressed act1-1-related salt sensitivity and the defect in actin repolarization of the rvs161{Delta} mutant, providing a link between sphingolipids and actin polarization. Indeed, deletion of the suppressor genes suppressed the rvs161{Delta} defect in actin repolarization in two ways: either actin was not depolarized at the wild-type level in a set of suppressor mutants, or actin was repolarized in the absence of Rvs161p in the other suppressor mutants. Rvs161p was localized as cortical patches that concentrated at polarization sites, i.e., bud emergence and septa, and was found to be associated with lipid rafts. An important link between sphingolipids and actin polarization is that Rvs161p was required for actin repolarization and was found to be located in lipid rafts.

* Corresponding author. Mailing address: IBGC/CNRS UMR 5095, 1 rue Camille Saint-Saëns, F-33077 Bordeaux Cedex, France. Phone: 33 5 56 99 90 53. Fax: 33 5 56 99 90 59. E-mail: Annick.Breton{at}ibgc.u-bordeaux2.fr.

Eukaryotic Cell, December 2002, p. 1021-1031, Vol. 1, No. 6
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.6.1021-1031.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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