Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murillo, L. A.
Right arrow Articles by Agabian, N. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murillo, L. A.
Right arrow Articles by Agabian, N. M.

 Previous Article  |  Next Article 

Eukaryotic Cell, September 2005, p. 1562-1573, Vol. 4, No. 9
1535-9778/05/$08.00+0     doi:10.1128/EC.4.9.1562-1573.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genome-Wide Transcription Profiling of the Early Phase of Biofilm Formation by Candida albicans{dagger}

Luis A. Murillo,1 George Newport,1 Chung-Yu Lan,1,{ddagger} Stefan Habelitz,2 Jan Dungan,1 and Nina M. Agabian1,3,4*

Departments of Cell and Tissue Biology,1 Preventive and Restorative Dental Sciences,2 Microbiology and Immunology,3 Pharmaceutical Chemistry, University of California, San Francisco, California 941434

Received 24 February 2005/ Accepted 10 June 2005

The ability to adhere to surfaces and develop as a multicellular community is an adaptation used by most microorganisms to survive in changing environments. Biofilm formation proceeds through distinct developmental phases and impacts not only medicine but also industry and evolution. In organisms such as the opportunistic pathogen Candida albicans, the ability to grow as biofilms is also an important mechanism for persistence, facilitating its growth on different tissues and a broad range of abiotic surfaces used in medical devices. The early stage of C. albicans biofilm is characterized by the adhesion of single cells to the substratum, followed by the formation of an intricate network of hyphae and the beginning of a dense structure. Changes in the transcriptome begin within 30 min of contact with the substrate and include expression of genes related to sulfur metabolism, in particular MET3, and the equivalent gene homologues of the Ribi regulon in Saccharomyces cerevisiae. Some of these changes are initiated early and maintained throughout the process; others are restricted to the earliest stages of biofilm formation. We identify here a potential alternative pathway for cysteine metabolism and the biofilm-associated expression of genes involved in glutathione production in C. albicans.

* Corresponding author. Mailing address: Department of Cell and Tissue Biology, University of California, San Francisco, 521 Parnassus Ave., San Francisco, CA 94143-0422. Phone: (415) 476-6845. Fax: (415) 476-0664. E-mail: agabian{at}itsa.ucsf.edu.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: Institute of Molecular and Cell Biology and Department of Life Sciences, National Tsing Hua University, Hsin-Chu, Taiwan.

Eukaryotic Cell, September 2005, p. 1562-1573, Vol. 4, No. 9
1535-9778/05/$08.00+0     doi:10.1128/EC.4.9.1562-1573.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2005 by the American Society for Microbiology.