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Eukaryotic Cell, April 2006, p. 781-783, Vol. 5, No. 4
1535-9778/06/$08.00+0     doi:10.1128/EC.5.4.781-783.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Giardia lamblia Attachment Force Is Insensitive to Surface Treatments

W. R. Hansen,1 O. Tulyathan,2 S. C. Dawson,3 W. Z. Cande,3 and D. A. Fletcher1,2*

Biophysics Graduate Group,1 Department of Bioengineering,2 Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California3

Received 20 December 2005/ Accepted 14 February 2006

Giardia lamblia cell populations show 90% detachment from glass under normal forces of 2.43 ± 0.33 nN applied by centrifugation. Detachment forces were not significantly different for cells attached to positively charged, hydrophobic, or inert surfaces than for cells attached to plain glass. The insensitivity of attachment force to surface treatment is consistent with a suction-based mechanism of attachment.

* Corresponding author. Mailing address: 459 Evans Hall no. 1762, UC Berkeley, Berkeley, CA 94720. Phone: (510) 643-5624. Fax: (510) 642-5835. E-mail: fletch{at}berkeley.edu.

Eukaryotic Cell, April 2006, p. 781-783, Vol. 5, No. 4
1535-9778/06/$08.00+0     doi:10.1128/EC.5.4.781-783.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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