Eukaryotic Cell
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Eukaryotic Cell, February 2008, p. 202-211, Vol. 7, No. 2
1535-9778/08/$08.00+0     doi:10.1128/EC.00292-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Excystation of Eimeria tenella Sporozoites Impaired by Antibody Recognizing Gametocyte/Oocyst Antigens GAM22 and GAM56{triangledown}

Jürgen Krücken,1* Ralf J. Hosse,1 Aimdip N. Mouafo,2 Rolf Entzeroth,2 Stefan Bierbaum,1 Predrag Marinovski,1 Karolina Hain,1 Gisela Greif,3 and Frank Wunderlich1

Division of Molecular Parasitology and Biological and Medical Research Centre, Heinrich-Heine-University, Düsseldorf,1 Institute of Zoology, TU-Dresden, Dresden,2 Animal Health Business Group, Research & Development, Bayer AG, Monheim, Germany3

Received 10 August 2007/ Accepted 14 November 2007

Eimeria tenella is the causative agent of coccidiosis in poultry. Infection of the chicken intestine begins with ingestion of sporulated oocysts releasing sporocysts, which in turn release invasive sporozoites. The monoclonal antibody E2E5 recognizes wall-forming body type II (WFBII) in gametocytes and the WFBII-derived inner wall of oocysts. Here we describe that this antibody also binds to the stieda body of sporocysts and significantly impairs in vitro excystation of sporozoites. Using affinity chromatography and protein sequence analysis, E2E5 is shown to recognize EtGAM56, the E. tenella ortholog of the Eimeria maxima gametocyte-specific GAM56 protein. In addition, this antibody was used to screen a genomic phage display library presenting E. tenella antigens as fusion proteins with the gene VIII product on the surfaces of phagemid particles and identified the novel 22-kDa histidine- and proline-rich protein EtGAM22. The Etgam22 mRNA is expressed predominantly at the gametocyte stage, as detected by Northern blotting. Southern blot analysis in combination with data from the E. tenella genome project revealed that Etgam22 is an intronless multicopy gene, with approximately 12 to 22 copies in head-to-tail arrangement. Conspicuously, Etgam56 is also intronless and is localized adjacent to another gam56-like gene, Etgam59. Our data suggest that amplification is common for genes encoding oocyst wall proteins.

* Corresponding author. Mailing address: Division of Molecular Parasitology, Heinrich-Heine-University, Universitätsstr. 1, 40225 Düsseldorf, Germany. Phone: 49-211-8114733. Fax: 49-211-8114734. E-mail: kruecken{at}uni-duesseldorf.de

{triangledown} Published ahead of print on 14 December 2007.

Eukaryotic Cell, February 2008, p. 202-211, Vol. 7, No. 2
1535-9778/08/$08.00+0     doi:10.1128/EC.00292-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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