Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Other Versions of this Article:
EC.00248-07v1
7/2/415    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Hallen, H. E.
Right arrow Articles by Trail, F.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hallen, H. E.
Right arrow Articles by Trail, F.
Eukaryotic Cell, February 2008, p. 415-424, Vol. 7, No. 2
1535-9778/08/$08.00+0     doi:10.1128/EC.00248-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum){triangledown} ,{dagger}

Heather E. Hallen1 and Frances Trail1,2*

Department of Plant Biology,1 Department of Plant Pathology, Michigan State University, East Lansing, Michigan 48824-13122

Received 10 July 2007/ Accepted 20 November 2007

Cch1, a putative voltage-gated calcium ion channel, was investigated for its role in ascus development in Gibberella zeae. Gene replacement mutants of CCH1 were generated and found to have asci which did not forcibly discharge spores, although morphologically ascus and ascospore development in the majority of asci appeared normal. Additionally, mycelial growth was significantly slower, and sexual development was slightly delayed in the mutant; mutant mycelia showed a distinctive fluffy morphology, and no cirrhi were produced. Wheat infected with {Delta}cch1 mutants developed symptoms comparable to wheat infected with the wild type; however, the mutants showed a reduced ability to protect the infected stalk from colonization by saprobic fungi. Transcriptional analysis of gene expression in mutants using the Affymetrix Fusarium microarray showed 2,449 genes with significant, twofold or greater, changes in transcript abundance across a developmental series. This work extends the role of CCH1 to forcible spore discharge in G. zeae and suggests that this channel has subtle effects on growth and development.

* Corresponding author. Mailing address: Department of Plant Biology, Michigan State University, East Lansing, MI 48824-1312. Phone: (517) 432-2939. Fax: (517) 353-1926. E-mail: trail{at}msu.edu

{triangledown} Published ahead of print on 14 December 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, February 2008, p. 415-424, Vol. 7, No. 2
1535-9778/08/$08.00+0     doi:10.1128/EC.00248-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2008 by the American Society for Microbiology.