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The Asexual Yeast Candida glabrata Maintains Distinct a and Haploid Mating Types

Institut Pasteur, Unité de Génétique Moléculaire des Levures, CNRS URA 2171, Université Pierre et Marie Curie—Paris 6 UFR927, F75015 Paris, France,¹ Université Pierre et Marie Curie—Paris 6 UMRS511, INSERM, Faculté de Médecine St.-Antoine, Parasitologie-Mycologie, F75012 Paris, France²

Received 20 December 2007/ Accepted 8 March 2008

The genome of the type strain of Candida glabrata (CBS138, ATCC 2001) contains homologs of most of the genes involved in mating in Saccharomyces cerevisiae, starting with the mating pheromone and receptor genes. Only haploid cells are ever isolated, but C. glabrata strains of both mating types are commonly found, the type strain being MAT and most other strains, such as BG2, being MATa. No sexual cycle has been documented for this species. In order to understand which steps of the mating pathway are defective, we have analyzed the expression of homologs of some of the key genes involved as well as the production of mating pheromones and the organism's sensitivity to artificial pheromones. We show that cells of opposite mating types express both pheromone receptor genes and are insensitive to pheromones. Nonetheless, cells maintain specificity through regulation of the 1 and 2 genes and, more surprisingly, through differential splicing of the a1 transcript.

Published ahead of print on 28 March 2008.