Eukaryotic Cell Visit the Journal of Bacteriology
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EC Accepts, published online ahead of print on 15 February 2008
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Eukaryotic Cell doi:10.1128/EC.00007-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evidence of a continuous endoplasmic reticulum in the protozoan parasite Entamoeba histolytica

Jose E. Teixeira and Christopher D. Huston*

Departments of Medicine, and Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont 05405

* To whom correspondence should be addressed. Email: christopher.huston{at}uvm.edu.


  Abstract

Entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted GFP-fusion protein and fluorescence loss in photobleaching (FLIP), we have unambiguously demonstrated the presence of a continuous ER compartment in living E. histolytica trophozoites.




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