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FIG. 4. Cell wall and newly synthesized capsule overlying nascent buds. Cell walls were tagged with fluorescein (green), and capsules were labeled with fluorophore-linked antibody to GXM (red). Cells were then returned to culture to allow bud emergence. A comparison of differential interference contrast (left) and fluorescence (right) images shows that none of the original label extends beyond the mother-bud neck (arrow), indicating that there is no incorporation of maternal glycans in the new bud. Reprinted with permission from Blackwell Publishing (80).
